Coding

Part:BBa_K322120

Designed by: Meng Lu and William Rostain   Group: iGEM10_Edinburgh   (2010-10-27)


Green light sensor with LacZ reporter

This design involves the fusion of CcaS and PhoR to create a green light sensor, by replacing the downstream portion of the CcaS with the downstream portion of PhoR based on the fact that both contain the same domains HisKA and HATPase-C. PhoR phosphorylates the response regulator PhoB, which then will binds to conserved DNA sequences called phoboxes and interacts with the &70 subunit of RNA polymerase to control transcription. Thus, we can add the reporter gene here as a response to the detection of green light.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2640
    Illegal SpeI site found at 2735
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2640
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 2011
    Illegal SpeI site found at 2735
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2640
    Illegal BamHI site found at 644
    Illegal XhoI site found at 2948
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2640
    Illegal SpeI site found at 2735
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2640
    Illegal SpeI site found at 2735
    Illegal NgoMIV site found at 971
    Illegal AgeI site found at 2675
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
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